List of Experiments / Activities
- BASIC BIOCHEMISTRY LAB
- 1Qualitative test of Carbohydrates
- 2Qualitative test of protein and amino-acids
- 3Quantitative Estimation of proteins
- 4Quantitative Estimation of glucose
- 5Paper chromatography
- 6To distinguish reducing sugar from non-reducing sugar
- 7To distinguish pentose from hexose
- 8To distinguish ketose from aldose
- 9To determine the presence of starch by iodine test
- 10To determine the presence of sucrose by acid base hydrolysis
- Note: The instructor may add/delete/modify/tune experiments; (*2 can be demonstration)
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Recall different carbohydrate tests and their principle.K1
- CO2Apply Lowry/Biuret method to determine protein concentration.K3
- CO3Analyze separation of amino acids/sugars based on Rf values, estimate concentration of biomoleculesK4
- CO4Understand principle of Benedict’s/Fehling’s test and interpret results.K2
- CO5Explain the iodine-starch complex formation and interpret the observed color change.K4
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