Computational Biology Lab

List of Experiments / Activities

1. COMPUTATIONAL BIOLOGY LAB
2. 1Knowledge of different biological database
3. •Protein and gene sequence databases (NCBI, DDBJ, EMBL, SWISS PROT, PIR)
4. •Structured at a bases (MMDB, PDB, CATH, SCOP)
5. •Pathway Databases (KEGG, BRENDA, METACYC)
6. •Bibliographic database (PUBMED, MEDLINE)
7. 2Sequence retrieval from biological database
8. 3To retrieve nucleotide sequences from GenBank in flat file format.
9. 4To identify and analyze various fields (LOCUS, DEFINITION, ACCESSION, FEATURES, ORIGIN) in a GenBank flat file.
10. 5Retrieve a protein structure (PDB file) from the Protein Data Bank (PDB) and visualize it using PyMOL, Chimera, or Jmol.
11. 6Identify alpha-helices, beta-sheets, and active sites.
12. 7Sequence similarity searching of nucleotide and protein sequences
13. 8Finding homologous sequences
14. 9Multiples Sequence alignment
15. 10Dynamic programming method-local and global alignment
16. 11Gene prediction methods
17. Note: The instructor may add/delete/modify/tune experiments B. TECH (BIOINFORMATICS)