List of Experiments / Activities
- BASIC MOLECULAR GENETICS LAB
- 1Preparation of buffers
- 2Preparation of different concentration of Agarose gel
- 3Horizontal Agarose gel electrophoresis
- 4Isolation of Plant genome
- 5Isolation of Bacterial genome
- 6Isolation of Plasmid DNA
- 7PCR amplification of DNA and visualization by gel electrophoresis
- 8Estimation of DNA by spectrophotometric method
- 9Estimation of Tm of duplex DNA strand using UV-Spectrophotometer
- 10Demonstration of DNA Sequencing
- Note: The instructor may add/delete/modify/tune experiments; (* two can be demonstration)
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Remember principles of DNA structure, sources (plasmid, plant, bacterial) and isolation basics.K3
- CO2Explain the principles of electrophoresis, PCR, and spectrophotometry in DNA analysis.K3
- CO3Perform DNA isolation, amplification, and quantification using standard protocols.K4
- CO4Interpret agarose gel patterns and PCR products,K4
- CO5Applying knowledge of absorbance to quantify DNA concentration.K3
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