List of Experiments / Activities
- 1Restriction enzyme digestion of plasmid
- 2Purification of digested DNA
- 3Ligation of DNA fragment with plasmid DNA
- 4Preparation of competent cells
- 5Transformation in E. coli
- 6Isolation of recombinant plasmid and confirmation of insert DNA
- 7Blue-white screening of transformants
- 8Polymerase Chain Reaction
- 9Sanger Sequencing
- 10Next Generation Sequencing
- Note: The instructor may add / delete / modify / tune experiments as required. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Apply restriction digestion, ligation, and PCR techniques for DNA manipulation.K3
- CO2Prepare competent cells, perform transformation in E. coli, and examine recombinant clones.K3
- CO3Isolate genomic and plasmid DNA, purify digested fragments, and confirm insert integration.K3
- CO4Examine recombinant constructs using agarose gel electrophoresis, PCR products, and blue-white screening.K3
- CO5Demonstrate practical skills in recombinant DNA technology workflows from DNA isolation to gene amplification.K3
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