Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked —
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List of Experiments / Activities
- Preparation of solutions: (i) Percentage solutions, (ii) Molar solutions, (iii) Normal solutions
- 2Titration of weak acid-weak base
- 3To perform quantitative estimation of carbohydrates
- 4To distinguish reducing and non-reducing sugars
- 5To perform quantitative estimation of proteins
- 6To estimate nucleic acids
- 7To perform isoelectric precipitation
- 8To separate sugars, fatty acids and amino acids by paper chromatography
- 9To extract lipids from plant material
- 10To perform thin layer chromatography
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Understand the basics of molarity, normality, buffers and pH meter.K2
- CO2Demonstrate the basics of titration and carbohydrate estimation.K4
- CO3Exhibit the difference between reducing and non-reducing sugars and estimation of proteins and nucleic acids.K4
- CO4Perform the extraction method of lipids and proteins on the basis of isoelectric point.K4
- CO5Understand the significance of chromatography.K4
Text books
- Wilson and Walker, “Principles and Techniques of Practical Biochemistry”, 4 Edn., Cambridge Knew pros 1997.
- Plummer DT, “An Introduction to Practical Biochemistry”, III Edn., Tata McGraw hill.
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