Unit 1: General Concepts
8 hrsVirus history, Diversity, shapes, sizes and components of genomes. Isolation and purification of viruses and components.
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Virus history, Diversity, shapes, sizes and components of genomes. Isolation and purification of viruses and components.
Consequences of virus infection to animals and human. Viral infection: affect on host macromolecules. Viral infection: establishment of the antiviral state. Viruses counter attack mechanisms. Viral diagnostic techniques: Rapid Antigen testing, RTPCR.
Classification of viruses and nomenclatures. +strand RNA viruses- Picorna viruses. Flavi viruses- West Nile virus and Dengue virus. Corona viruses- SARS pathogens. Small DNA viruses: parvo- and polyoma viruses. Large DNA viruses: Herpes-adeno-, and poxviruses. Miscellaneous viruses.
–ve strand RNA viruses Paramyxo viruses. Orthomyxo viruses: Influenza pathogenesis and Bird flu. Rhabdo viruses: Rabies pathogenesis.. dsRNA viruses- Reo viruses. Retroviruses: structure, classification, life cycle; reverse transcription. Retroviruses: HIV, viral pathogenesis and AIDS.
Antivirals and viral vaccines Viral Vaccines Conventional vaccines-killed and attenuated, modern vaccinesrecombinant proteins, subunits, DNA vaccines, peptides, immunemodulators (cytokines), vaccine delivery and adjuvants, large scale manufacturing- QA/QC issues. Antivirals Interferons, designing and screening of antivirals, mechanism of action, antiviral libraries, antiretrovirals- mechanism of action and drug resistance. Modern approaches of virus control Anti-sense RNA, siRNA, ribozymes.
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