UNIQUE SERIES AKTU Syllabus
BP207P

Biochemistry (Practical)

L-T-P0-0-3 Credits1 Session2026-27 TypeLab / Practical
Appears in 1 semester

B.Pharm · Sem 2 (Lab)

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List of Experiments / Activities

  1. Biochemistry (Practical) Core Credit Hours Per Week (L-T-P) Max. Hours L T P
  2. 1-- --
  3. 45Maximum Marks SE ESE
  4. (Minimum 12experiments must be performed)
  5. 1Identification tests for proteins (Albumin and Casein).
  6. 2Qualitative analysis of carbohydrates* (Glucose, Fructose, Lactose, Sucrose, and Starch).
  7. 3Qualitative analysis of urine for normal and abnormal constituents.
  8. 4Estimation of blood glucose.
  9. 5Estimation of total cholesterol and HDL cholesterol.
  10. 6Estimation of urea, creatinine, and uric acid in serum.
  11. 7Estimation of serum total protein and albumin.
  12. 8Study of enzymatic hydrolysis of starch.
  13. 9Study of the effect of temperature on salivary amylase activity.
  14. 10Study of the effect of substrate concentration on salivary amylase activity.
  15. 12Estimation of serum bilirubin (total and direct bilirubin).
  16. 13Estimation of glycogen content
  17. 14Estimation of SGOT and SGPT
Course Objectives6 points
  • The objectives of this course are:
  • To develop skills to identify and differentiate carbohydrates and proteins through classical qualitative biochemical tests.
  • To apply biochemical techniques for analysis of pathological conditions using urine and blood samples.
  • To perform estimations of clinically important biomolecules such as glucose, cholesterol, urea, creatinine, uric acid, and proteins in biological fluids.
  • To demonstrate enzymatic activity and study factors affecting enzyme function, including substrate concentration and temperature.
  • To interpret biochemical results and correlate them with clinical conditions such as diabetes, renal dysfunction, and lipid disorders.
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Identify proteins and carbohydrates using qualitative biochemical tests and explain their physiological relevance.
  2. CO2Detect normal and abnormal constituents in urine and interpret their diagnostic significance.
  3. CO3Estimate and interpret levels of glucose, cholesterol, urea, creatinine, uric acid, and serum proteins, and correlate results with clinical conditions.
  4. CO4Demonstrate enzyme substrate reactions and evaluate the effect of temperature and substrate concentration on enzymatic activity.
  5. CO5Analyze, record, and report biochemical results accurately and relate practical findings to theoretical knowledge and clinical application.
Recommended References (Preferably latest editions):6 books
  1. Plummer, D. T. An Introduction to Practical Biochemistry. McGraw-Hill, New York.
  2. Wilson, K., and Walker, J. Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press.
  3. Varley, H., Gowenlock, A. H., Bell, M., and Bell, J. L. Biochemistry. Heinemann Medical Books.
  4. Burtis, C. A., Ashwood, E. R., and Bruns, D. E. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Elsevier.
  5. Rodwell, V. W., Bender, D. A., Botham, K. M., Kennelly, P. J., and Weil, P. A. McGraw-Hill.
  6. Freifelder, D. Essential Molecular Biology: A Practical Approach. Oxford University Press. Course Title Course Type

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Official AKTU syllabus

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Official AKTU syllabus PDF ↗