UNIQUE SERIES AKTU Syllabus
BP408P

Pharmaceutical Biotechnology (Practical)

L-T-P0-0-3 Credits1 Session2026-27 TypeLab / Practical
Appears in 1 semester

B.Pharm · Sem 4 (Lab)

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List of Experiments / Activities

  1. Pharmaceutical Biotechnology (Practical) Core Credit Hours Per Week (L-T-P) Max. Hours L T P
  2. 1-- --
  3. 45Maximum Marks SE ESE
  4. (Minimum 12 experiments must be performed)
  5. 1Understanding Good microbiological laboratory practices while working in Microbiology laboratory aseptic handling in microbiology lab: growth and isolation of microbes using streaking, spreading and subculturing techniques: study of various equipments such as loop, straight wire, spreader, forceps, pipette, test tube, petridish, burner etc and apparatus used in microbiology lab- BOD incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer and microscopes.
  6. 2Handling of biological spill, decontamination procedures and hygiene while handling microorganisms and disposal
  7. 3Sterilization and evaluation of glassware and apparatus using hot air oven
  8. 4Preparation and sterilization of solid and liquid culture medium using different techniques
  9. 5Determination of microbiological efficacy of disinfectant/ preservative efficacy test
  10. 6Tests for sterility of ophthalmic or parenteral marketed formulation according to IP.
  11. 7Analysis of Biotechnological product (Protein, nucleic acid materials) by UV Vis and FTIR spectrophotometer
  12. 8Production of alcohol using Fermentation process
  13. 9Practice Whole cell immobilization technique (any one)
  14. 10Practice Enzyme immobilization technique and its kinetics
  15. 11Isolation and estimation of DNA and RNA
  16. 12Isolation of plasmids and expression of protein
  17. 13Agarose gel electrophoresis of DNA/ RNA
  18. 14SDS polyacrylamide gel electrophoresis for proteins.
  19. 15Microbiological assay of antibiotics.
Course Objectives6 points
  • The objectives of this course are to:
  • Provide fundamental knowledge of biotechnology concepts, including protein therapeutics, monoclonal antibodies, enzyme immobilization, and their pharmaceutical applications.
  • Explain the principles and applications of genetic engineering and recombinant DNA technology in pharmaceutical sciences.
  • Develop understanding of microbial biotransformation, fermentation processes, and large-scale production of pharmaceutical products such as alcohol, antibiotics, vaccines, and blood products.
  • Describe the principles, methodologies, delivery systems, and ethical considerations associated with gene therapy and modern vaccine technologies.
  • Introduce the regulatory requirements and standardization procedures for vaccines, sera, and other biopharmaceutical products.
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Explain the fundamental principles of biotechnology and apply genetic engineering and recombinant DNA technology concepts in pharmaceutical sciences.
  2. CO2Describe the production and applications of biopharmaceutical products such as protein therapeutics and monoclonal antibodies.
  3. CO3Explain the principles, delivery systems, and ethical considerations associated with gene therapy.
  4. CO4Describe fermentation processes and microbial biotransformation involved in the large-scale production of pharmaceutical products such as vaccines, hormones, and blood products.
  5. CO5Explain the principles of immunization, types of vaccines and sera, and the regulatory and ethical considerations involved in the development of biopharmaceutical products.
Recommended References (Preferably latest editions):7 books
  1. Baltz, R. H., Davies, J. E. and Demain, A. L. Manual of Industrial Microbiology and Biotechnology. ASM Press.
  2. Carter, S. J. CBS Publishers & Distributors.
  3. Chou, S. L. M. Practical Biotechnology: A Guide to Biochemical Engineering. Springer.
  4. Denyer, S. P., Hodges, N. A. and Gorman, S. P. Microbiology. Wiley-Blackwell.
  5. Glick, B. R., Pasternak, J. J. and Patten, C. L. Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press.
  6. Lachman, L., Lieberman, H. A., Kanig, J. L. and Khar, R. K. The Theory and Practice of Industrial Pharmacy. CBS Publishers & Distributors.
  7. Pelczar, M. J., Chan, E. C. S. and Krieg, N. R. Microbiology. McGraw-Hill. Course Title Course Type

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Official AKTU syllabus

This subject is on page 113, 114, 115 of the original AKTU PDF.

Official AKTU syllabus PDF ↗