Systemic Pharmacology I (Practical)

List of Experiments / Activities

1. Systemic Pharmacology I (Practical) Core Credit Hours Per Week (L-T-P) Max. Hours L T P
2. 1-- --
3. 45Maximum Marks SE ESE
4. (Minimum 12 Experiments must be performed)
5. 1To demonstrate Langendorff’s heart assembly and its application in pharmacology (only video demonstration/or charts and illustrations).
6. 2Recording of the effects of different electrolytes, agonists and antagonists on the isolated and perfused frog heart preparation using interactive computer simulation experiment.
7. 3To study the effect of various drugs on blood pressure and heart rate anaesthetized dog using interactive computer simulation experiment.
8. 4Demonstration of the estimation of intraocular pressure on rabbit eye and human eye by using conventional Schiotz tonometer and non-contact tonometer.
9. 5To evaluate the muscle relaxant activity of drugs on Rota-rod apparatus using interactive computer simulation experiment.
10. 6Demonstration of the effect of spasmogens and spasmolytic using rabbit jejunum using interactive computer simulation experiment.
11. 7Determination of PA2 value of Atropine using a suitable isolated tissue preparation by Schild plot plot method with the help of hypothetical data using interactive computer simulation experiment.
12. 8Determination of PA2 value of Prazosin using a suitable isolated tissue preparation by Schild method with the help of hypothetical data using interactive computer simulation experiment.
13. 9To study the antiallergic effects of drugs using mast cell degranulation assay using interactive computer simulated experiment
14. 10Evaluation of diuretic activity of drugs in rats using metabolic cages (simulation) and estimation of electrolytes in the urine samples/serum using flame photometer/commercially available kits.
15. 11Evaluation of effects of antihistaminic drugs on the histamine-induced bronchospasm in guinea pigs using interactive simulated experiment.
16. 12To study and analyse drug-drug interaction/ rational drug management of asthma with the help of Case Study/ hypothetical data/ any clinical report.
17. 13To study clinical pharmacology of the plasma volume expanders and their importance (using charts/open sources videos).
18. 14Demonstration of evaluation of anti-inflammatory activity in paw oedema model using plethysmometer using simulations.
19. 15Concept of Biobanking and its significance in drug screening.
20. 16Isolation of Polymorphonuclear cells from blood and evaluation of endotoxin-induced oxidative stress in them using Nitrobluetetrazolium dye method by colourimetry.
21. 17Determination of viability of cells (using isolated polymorphonuclear cells) by Trypan blue dye exclusion assay to determine cell viability using microscopy method.
22. 18Determination of anti-inflammatory activity of durgs in vitro using RBC membrane stabilization assay
23. 19Determination of protein concentrations using Bradford reagent and plotting of standard curve of bovine serum albumin.
24. 20Estimation of anticholinesterase inhibitory activity of drugs using Ellman’s reagent (DTNB)
25. 21Determination and and plotting of standard curve for LPO, Nitric oxide and GSH.