Modern Analytical Techniques (Theory)

• Nuclear Magnetic Resonance Spectroscopy: Principles of ¹H-NMR and ¹³C-NMR, various solvents used, chemical shift, factors affecting chemical shift, coupling constant, spin spin coupling, relaxation, instrumentation of FT-NMR and its applications.
• Mass Spectrometry: Principles, fragmentation and its rules, ionization techniques Electron impact, chemical ionization, MALDI, FAB, API, analyzers Time of flight and quadrupole, ion trap, detectors and applications.

• X-Ray Diffraction Methods: Origin of X-Rays, basic aspects of crystals, X-Ray crystallography, rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation and applications.
• Thermal Analysis: Introduction, instrumentation, factors affecting measurements, applications of TGA, DSC (types) and DTA.

• UPLC and Nano LC: Principle, advantages over LC and applications.
• Principle and applications of hyphenated techniques: GC-MS, LC- MS/MS, ICP-MS.
• Supercritical chromatography and flash chromatography: principles and applications.

• Green Analytical Chemistry: Types of green solvents, various computational tools used to assess the greenness and its applications in sample preparation and analytical method development.
• Bio-analytical Methods: Introduction to bioanalytical method development, extraction of drugs and metabolites from biological fluids SPE, LLE, PPE, BCS classification, PK-PD interaction, microsomal assays, MTT assay, BA & BE study protocol, biosimilars.
• Radio Immune Assays and ELISA: Importance, various components, principle, different methods, limitations and applications of radio immunoassay and ELISA.

Microscopy-Based Analytical Techniques: Principle, instrumentation, and applications of optical microscopy, scanning electron microscopy and transmission electron microscopy.