Modern Analytical Techniques (Practical)

• The objectives of this course are to:
• Provide practical training in interpretation of advanced instrumental data used for structural and physicochemical characterization.
• Introduce quantitative analytical techniques using modern separation methods, such as UHPLC and HPLC, for the estimation of pharmaceutical substances.
• Familiarize students with the principles and practice of green analytical chemistry, including preparation of green solvents and development of environmentally sustainable analytical methods.
• Develop understanding of bioanalytical sample preparation techniques for pharmaceutical analysis in biological matrices, including solid phase extraction, liquid liquid extraction, and protein precipitation.
• Learn applied pharmaceutical analysis and methods including cell viability assays, residual solvent analysis, and estimation of drugs from biological fluids.

After completion of the course student will be able to:

1. CO1Analyze and interpret proton NMR, carbon NMR, mass spectra, X-Ray diffraction patterns, and DSC thermograms to characterize pharmaceutical compounds.
2. CO2Perform quantitative analysis of official pharmaceutical compounds using UHPLC.
3. CO3Design and assess the suitability of green analytical solvents for pharmaceutical applications, promoting sustainability.
4. CO4Construct a comprehensive protocol for bioavailability and bioequivalence studies adhering to USFDA regulatory standards.
5. CO5Employ appropriate extraction techniques (SPE, PPE, LLE) for the accurate quantification of pharmaceuticals in biological fluids and matrices and explain and execute the MTT assay for evaluating cell viability in a laboratory setting.

1. Beckett, A. H. and Stenlake, J. B. Practical Pharmaceutical Chemistry. CBS Publishers & Distributors.
2. Brittain, H. G. Analytical Profiles of Drug Substances and Excipients. Elsevier.
3. Kemp, W. Organic Spectroscopy. Palgrave Macmillan.
4. Munson, J. W. Pharmaceutical Analysis: Modern Methods. Marcel Dekker.
5. Sethi, P. D. Quantitative Analysis of Drugs in Pharmaceutical Formulations. CBS Publishers & Distributors.
6. Sharma, B. K. Instrumental Methods of Chemical Analysis. Goel Publishing House.
7. Sharma, Y. R. Organic Spectroscopy. S. Chand.
8. Silverstein, R. M., Webster, F. X., Kiemle, D. J. and Bryce, D. L. Spectrometric Identification of Organic Compounds. Wiley.
9. Willard, H. H., Merritt, L. L., Dean, J. A. and Settle, F. A. Instrumental Methods of Analysis. Brooks/Cole.
10. Indian Pharmacopoeia. Indian Pharmacopoeia Commission. Course Title Course Type BP710RP Research Project CORE Credit Hours Per Week (L-T-P)* Max. Hours. L T P
11. -- -- -- -- Maximum Marks SE ESE 150
12. 150 * Refer section 21 of regulation.